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Physicochemical characterization of Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( A and B ) Diameter distribution and zeta potential measured by dynamic light scattering (DLS; n=3). ( C ) Cumulative dacarbazine (DTIC) release profile from nanoparticles in phosphate-buffered saline quantified by <t>liquid</t> <t>chromatography–mass</t> <t>spectrometry</t> (LC-MS; n=3). ( D ) Stability of nanoparticles in NS over 24 h assessed by DLS (n=3). ( E ) Representative scanning electron microscopy (SEM) images (scale bars = 200 nm). Data are expressed as mean ± standard deviation (SD). ( F ) SDS-PAGE protein analysis of CCM (Cancer cell membrane), Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( G ) Western blot analysis for the expression of membrane functional protein markers.
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Physicochemical characterization of Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( A and B ) Diameter distribution and zeta potential measured by dynamic light scattering (DLS; n=3). ( C ) Cumulative dacarbazine (DTIC) release profile from nanoparticles in phosphate-buffered saline quantified by liquid chromatography–mass spectrometry (LC-MS; n=3). ( D ) Stability of nanoparticles in NS over 24 h assessed by DLS (n=3). ( E ) Representative scanning electron microscopy (SEM) images (scale bars = 200 nm). Data are expressed as mean ± standard deviation (SD). ( F ) SDS-PAGE protein analysis of CCM (Cancer cell membrane), Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( G ) Western blot analysis for the expression of membrane functional protein markers.

Journal: International Journal of Nanomedicine

Article Title: Membrane-Coated Iron Oxide (Fe 3 O 4 ) Nanoparticles Encapsulating Dacarbazine Suppress B16F10 Melanoma Cell Activity in vitro and in a Subcutaneous Mouse Model

doi: 10.2147/IJN.S635477

Figure Lengend Snippet: Physicochemical characterization of Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( A and B ) Diameter distribution and zeta potential measured by dynamic light scattering (DLS; n=3). ( C ) Cumulative dacarbazine (DTIC) release profile from nanoparticles in phosphate-buffered saline quantified by liquid chromatography–mass spectrometry (LC-MS; n=3). ( D ) Stability of nanoparticles in NS over 24 h assessed by DLS (n=3). ( E ) Representative scanning electron microscopy (SEM) images (scale bars = 200 nm). Data are expressed as mean ± standard deviation (SD). ( F ) SDS-PAGE protein analysis of CCM (Cancer cell membrane), Fe 3 O 4 NPs, Fe 3 O 4 P NPs, Fe 3 O 4 PD NPs, and Fe 3 O 4 PD@CCM NPs. ( G ) Western blot analysis for the expression of membrane functional protein markers.

Article Snippet: The physicochemical characteristics of the nanoparticles were systematically characterized using dynamic light scattering (DLS; Zetasizer Ultra, Malvern Panalytical, UK), scanning electron microscopy (SEM; Apreo 2, Thermo Fisher Scientific, USA), and liquid chromatography–mass spectrometry (LC-MS; LCMS-8060, Shimadzu, Japan).

Techniques: Zeta Potential Analyzer, Dynamic Light Scattering Assay, Saline, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy, Stability, Scanning Electron Microscopy, Standard Deviation, SDS Page, Analysis, Membrane, Western Blot, Expressing, Functional Assay